determination of volatile glucosinolate degradation products in seed coat, stem and in vitro cultures of moringa peregrina (forssk.) fiori.
نویسندگان
چکیده
moringaceae, a monogeneric family in capparales (glucosinolate-containing species), includes 14 species. one of them is moringa peregrina (forssk.) fiori., a small tree, which grows in south east of iran. volatile constituents of seed coat and stem of m. peregrina were determined by gc and gc/ms. moreover, extracts of seed and different cultured cells were analyzed by tlc and gc. three volatile isothiocyanates including isopropyl isothiocyanate (4.2%), sec-butyl isothiocyanate (< 0.1%) and isobutyl isothiocyanate (92.9%) were found in the volatile oil of the stem , while only two volatile isothiocyanates namely isopropyl isothiocyanate (7.0%) and isobutyl isothiocyanate (51.5%) were determined in the seed coat of the tree. for the first time, the callus and suspension cultures of m. peregrina were initiated and established successfully on murashige and skoog medium, containing plant growth hormones. different precursors and elicitors were fed to the cultures to induce glucosinolates production. this is the first report of in vitro culture production of m. peregrina. there was no production of volatile isothiocyanates in m. peregrina callus and suspension cultures with different treatments.
منابع مشابه
Determination of Volatile Oil Constituents in Root and Shoot of Moringa peregrina (Forssk.) Fiori in Saline Soil
Background: Moringa peregrina (Forssk.) Fiori. is a small tree, which grows in south east of Iran. The shoot and root of M. peregrina contain volatile oil which has several isothiocyanates. Objective: To study the effect of salinity on volatile constituents of root and shoot of M. peregrina. Methods: The experiment was conducted on randomized complete blocks design (RCBD) with 8 treatments...
متن کاملVolatile Constituents of the Seed Kernel and Leaf of Moringa peregrina (Forssk.) Fiori, Agricolt. Cultivated in Chabahar (Iran)
Volatile constituents of the seed kernel and leaf of cultivated Moringa peregrina (Forssk.) Fiori, Agricolt collected after hydrolysis were analyzed by GC and GC/MS. Five glucosinolate degradation products which constituted almost the whole isolated oil of the seed kernel were identified to be: isobutyl isothiocyanate (94.0%), isopropyl isothiocyanate (4.9%), sec-butyl isothiocy...
متن کاملvolatile constituents of the seed kernel and leaf of moringa peregrina (forssk.) fiori, agricolt. cultivated in chabahar (iran)
volatile constituents of the seed kernel and leaf of cultivated moringa peregrina (forssk.) fiori, agricolt collected after hydrolysis were analyzed by gc and gc/ms. five glucosinolate degradation products which constituted almost the whole isolated oil of the seed kernel were identified to be: isobutyl isothiocyanate (94.0%), isopropyl isothiocyanate (4.9%), sec-butyl isothiocyanate (0.5%), n-...
متن کاملdetermination of volatile oil constituents in root and shoot of moringa peregrina (forssk.) fiori in saline soil
background: moringa peregrina (forssk.) fiori. is a small tree, which grows in south east of iran. the shoot and root of m. peregrina contain volatile oil which has several isothiocyanates. objective: to study the effect of salinity on volatile constituents of root and shoot of m. peregrina. methods: the experiment was conducted on randomized complete blocks design (rcbd) with 8 treatments and ...
متن کاملvolatile constituents of the seed kernel and leaf of moringa peregrina (forssk.) fiori, agricolt. cultivated in chabahar (iran)
متن کامل
Biochemical and Growth Responses of Moringa peregrina (Forssk.) Fiori to Different Sources and Levels of Salinity
Background: As Moringa peregrina is a valuable medicinal plant in traditional medicine, it is necessary to determine responses of this plant to salinity. Objective: To determine some biochemical and growth responses of Moringa peregrina to salinity at the seedling stage. Methods: This experiment was conducted in Institute of Medicinal Plants-ACECR, on base of factorial experiment as ...
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عنوان ژورنال:
research in pharmaceutical sciencesجلد ۷، شماره ۱، صفحات ۵۱-۰
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